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Cerberus Services 

 

Cerberus Sciences provides a wide range of serology, pathology and molecular diagnostic services to the Laboratory Animal community. Details of these services can be found by following the links on the navigation panel to the right. If you can't find what you are looking for, then please don't hesitate to contact us.


Serology 

EIA $14.00 per assay  (ex GST)     IFA $22.00 per assay (ex GST)

Abbreviation Agent Species Assay
CAR Cilia Associated Respiratory Bacillus Mouse, Rat, G Pig, Rabbit EIA/IFA
ECT Ectromelia Virus Mouse EIA/IFA
ECUN Encephaltozoon cuniculi Mouse, Rat, G Pig, Rabbit EIA/IFA
HAN Hantaan Virus Mouse, Rat EIA/IFA
K K Virus (mouse pneumonitis) Mouse EIA
KRV Kilham Rat Virus (a parvovirus of rats) Rat IFA
LCM Lymphocytic Choriomeningitis Virus Mouse, Rat EIA/IFA
MAD 1 Mouse Adenovirus Type 1 Mouse EIA/IFA
MAD 2 Mouse Adenovirus Type 2 Mouse EIA/IFA
MCMV Murine Cytomegalovirus Mouse EIA/IFA
MHV Mouse Hepatitis Virus (a mouse coronavirus) Mouse EIA/IFA
MNV Mouse Norovirus Mouse EIA/IFA
MPV Mouse Parvovirus (a mouse parvovirus rVP2 antigen) Mouse EIA/IFA
MTV Mouse Thymic Virus Mouse IFA
MVM Minute Virus of Mice (a mouse parvovirus) Mouse EIA/IFA
PARV Parvovirus (rNS1 generic assay) Mouse, Rat EIA
PI 3 Guinea Pig Parainfluenza Virus G Pig IFA
POLY Polyoma Virus Mouse EIA/IFA
PULM Mycoplasma Pulmonis Mouse, Rat EIA/IFA
PVM Pneumonia Virus of Mice Mouse, Rat, G Pig, Rabbit EIA/IFA
RCV/SDAV Rat Coronavirus/Sialodacryoadenitis Virus Rat EIA/IFA
ROTA Rotavirus (E.D.I.M.) Mouse EIA/IFA
RPV Rat Parvovirus Rat IFA
SEND Sendai Virus Mouse EIA/IFA
SV 5 Simian Virus 5 G Pig EIA/IFA
TH 1 Toolan's Virus (a parvovirus of rats) Rat IFA
TMEV Theiler's Murine Encephalomyelitis Virus (GD VII) Mouse, Rat EIA/IFA
TYZ Clostridium piliforme (Tyzerr's agent) Mouse, Rat EIA/IFA

 

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Serology Panels 

Abbreviation Species Tests Performed Notes Price*
AQIS Mouse, Rat HAN  Testing required for AQIS clearance of imported animals. A customised AQIS report is provided. $14.00
GP G Pig PVM, SEND, TYZ, ECUN, SV5, LCM, REO  Guinea Pig Panel $80.00
M1 Mouse MHV, MVM, MPV, MNV, ROTA, TMEV (6 agents) Virus pathogens most frequently detected by serology. Suggested monthly or quarterly screen. $70.00
M2 Mouse M1 panel plus PVM, SEND, PULM (9 agents) Most common respiratory pathogens included.
Suggested quarterly screen.
$100.00
M3 Mouse M2 panel plus MCMV, MAD1, REO, LCMV, ECT
(14 agents)
Suggested Bi-annual or annual screen. $160.00
M4 Mouse M3 panel plus MAD2, POLY, TYZ (17 agents)
 
Possible annual screen for strict barrier facilities. $195.00
M5 Mouse M4 panel plus HAN, ECUN, CARB, K, MTV (22 agents) Comprehensive panel :  May be necessary for exports or imports from suspect sources. $250.00
R1 Rat RCV/SDAV, PARV, PVM, PULM (4 agents) Pathogens most frequently detected in rats by serology. Suggested monthly screen. $50.00
R2 Rat R1 panel plus SEND, TMEV, REO, LCM (8 agents)  Suggested Quarterly screen $95.00
R3 Rat R2 panel plus MAD1, HAN, CAR, TYZ (12 agents) Suggested Bi-annual or annual screen $140.00
R4 Rat R3 panel plus MAD2, ECUN, RMV (15 agents) -  Suggested annual or export screen $170.00
RB Rabbit PVM, SEND, TYZ, ECUN, SV5, CAR   $70.00

*all prices GST exclusive.

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Pathology ( Animal Submissions) 

Cerberus Sciences offers a range of  animal monitoring and disease investigation packages incorporating serology, bacteriology, parasitology and histopathology.

Abbrev Description Price*
(per animal)
HMP-BASIC

Please Note – Histopathology and Serology are NOT included in the HMP-BASIC package.

Health monitoring package combining bacteriology, parasitology and molecular diagnostics.


External examination for lesions and ectoparasites (Tape test of pelage).

Euthanasia and sample collection for serology if required (-additional costs applied).

Necropsy and description of lesions found. If lesions are present, samples will be taken for further analysis. Submitter will be contacted for approval to proceed - Histopathology charged seperately by number of slides processed.

Nasotracheal wash taken. Nasotracheal wash retained for Mycoplasma PCR if required

Bacteriology culture of NT wash (CO2) and caecal contents (Aerobic) for detection of: B.bronchiseptica, Citrobacter rodentium Corynebacterium kutscheri, Klebsiella spp, Pasteurella spp, Pseudomonas spp, Proteus spp, Salmonella spp, Streptobacillus moniloformis, Staphylococcus (coag +ve), Streptococci (β haem), Streptococcus pneumoniae.

PCR if indicated by culture plates for: Citrobacter rodentium, Pasteurella spp, Pasteurella pneumotropica

Flush through and gross examination of intestinal contents for helminths.

Cellophane tape test for pinworm eggs

Wet mount preparation of GI mucosal scrapes for protozoa including Eimeria spp, Spironucleus spp, Giardia spp, Trichomonas spp.

Faecal flotation test for detection of pinworm (Syphacia spp and Aspiculuris spp), other worms and eggs.

 

$115.00
HMP-FULL

Health monitoring package/Disease Investigation combining bacteriology, parasitology, molecular diagnostics and histopathology.


External examination for lesions and ectoparasites (Tape test of pelage).

Euthanasia and sample collection for serology if required.

Necropsy and description of lesions found. If lesions are present, samples will be taken for further analysis.

Nasotracheal wash taken. Nasotracheal wash retained for Mycoplasma PCR if required

Bacteriology culture of NT wash (CO2) and caecal contents (Aerobic) for detection of: B.bronchiseptica, Citrobacter rodentium Corynebacterium kutscheri, Klebsiella spp, Pasteurella spp, Pseudomonas spp, Proteus spp, Salmonella spp,  Streptobacillus moniloformis, Staphylococcus (coag +ve), Streptococci (β haem), Streptococcus pneumoniae.

PCR if indicated by culture plates for: Citrobacter rodentium, Pasteurella spp, Pasteurella pneumotropica

Flush through and gross examination of intestinal contents for helminths.

Cellophane tape test for pinworm eggs

Wet mount preparation of GI mucosal scrapes for protozoa including Eimeria spp, Spironucleus spp, Giardia spp, Trichomonas spp.

Faecal flotation test for detection of pinworm (Syphacia spp and Asiculuris spp), other worms and eggs

Histology (Mice): Kidney, Liver, Lung, Stomach, Duodenum, Ileum, Caecum, Ascending Colon - further samples will be taken if lesions noted on necropsy

Histology (Rats): Kidney, Liver, Lung+Trachea, Salivary Gland, Harderian Gland, Bladder, Stomach, Duodenum, Caecum, Ascending colon.

Collection of additional samples for follow up testing/diagnosis if required (samples retained for one month prior to discard): lung+trachea, spleen,  mesenteric lymph node, liver,  caecum and salivary gland.
 

 $150.00
SBS-M

Package for Immunocompetent mice used as soiled bedding sentinels combining bacteriology, parasitology, serology and molecular diagnostics.

Please Note: Soiled bedding sentinels are most effective when used to detect organisms spread by the oral/faecal route and have been shown to be less effective in the detection of organisms spread by aerosol and contact. This package concentrates on organisms most likely to be transmitted by the oral/faecal route.

All testing as per the HMP-BASIC package

Plus

Serology: M1 Panel (MHV, MPV, MVM, ROTA, TMEV and MNV)

PCR of Caecal contents for Helicobacter spp (speciation can be carried out on request)

 

$210.00
SBS-R

Package for Immunocompetent rats used as soiled bedding sentinels combining bacteriology, parasitology, serology and molecular diagnostics.

Please Note: Soiled bedding sentinels are most effective when used to detect organisms spread by the oral/faecal route and have been shown to be less effective in the detection of organisms spread by aerosol and contact. This package concentrates on organisms most likely to be transmitted by the oral/faecal route.

All testing as per the HMP-BASIC package

Plus

Serology: R1 Panel (Parvovirus, RCV/SDAV. TMEV, REO3)

PCR of Caecal contents for Helicobacter spp (speciation can be carried out on request)


 

$195.00
FEL-MQ

Quarterly Health Monitoring for Mice as recommended by FELASA: Immunocompetent or sentinel animals only

Viruses: Serology - MVM; MPV; MHV; EDIM; PVM; SEND; TMEV; MNV

Bacteria: Serology - M.pulmonis, Clostridium piliforme

Culture - C.rodentium, Corynebacterium kutscheri, Pasteurella spp, Salmonella pp, Streptococci (β-haemolytic), Streptococcus pneumoniae

Parasites: Tape test - Ectoparasites, Syphacia spp

Wet mount/faecal float - Protozoa, pinworm, other worms and eggs.

Pathology: Pathological lesions observed
 

$240.00
FEL-MA

Annual Health Monitoring for Mice as recommended by FELASA:

FELASA MQ plus

Viruses: Serology – REO 3, ECT, MAD 1, MAD 2, MCMV, LCMV

Bacteria ; PCR – Helicobacter spp

Culture – Streptobacillus moniloformis
 

$325.00
FEL-RQ

Quarterly Health Monitoring for Rats as recommended by FELASA: Immunocompetent or sentinel animals only

Viruses: Serology - PVM; SEND; KRV, TH-1, RCV/SDAV, RPV

Bacteria: Serology - M.pulmonis, Clostridium piliforme

Culture - Corynebacterium kutscheri, Pasteurella spp, Salmonella pp, Streptococci (β-haemolytic), Streptococcus pneumoniae, Streptobacillus moniloformis

Parasites: Tape test - Ectoparasites, Syphacia spp
Wet mount/faecal float - Protozoa, pinworm, other worms and eggs.

Pathology: Pathological lesions observed
 

$220.00
FEL-RA

Annual Health Monitoring for Rats as recommended by FELASA:

FELASA RQ plus

Viruses: Serology – REO 3, HAN, MAD 1, MAD 2

Bacteria: PCR – Helicobacter spp
 

$290.00

*all prices GST exclusive.

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Molecular Diagnostics 

Cerberus Sciences offers a wide range of PCR and RT-PCR assays for the detection of viruses and other pathogens in tissues, cell culture and other biological materials.

PCR

1st assay performed on a sample $55.00*. Subsequent assays performed on the same sample $27.50*.

RT-PCR

1st assay performed on a sample $66.00*. Subsequent assays performed on the same sample $27.50*.

Type Agent Tissue Sample Type Required
PCR (Bacteria) Mycoplasma species (sub typing to M. pulmonis available) Trachea, Nasopharynx
PCR (Bacteria) Helicobacter species Faeces, Caecum
PCR (Bacteria) Helicobacter hepaticus Faeces, Caecum
PCR (Bacteria) Helicobacter bilis Faeces, Caecum
PCR (Bacteria) Helicobacter muridarum Faeces, Caecum
PCR (Bacteria) Helicobacter rodentium Faeces, Caecum
PCR (Bacteria) Pasteurella pneumotropica (sub typing to Jawetz and Heyl available) Nasopharynx
PCR (Bacteria) Salmonella spp Faeces. Caecum
PCR (Bacteria) Citrobacter rodentium Faeces, Caecum
PCR (Bacteria) Clostridium piliforme (Tyzerr's agent) Caecum
PCR (Fungi) Pneumocystis carinii Lung
PCR (DNA Viruses) Minute Virus of Mice Intestine, faeces
PCR (DNA Viruses) Mouse parvovirus Intestine, faeces
PCR (DNA Viruses) Rat parvovirus Intestine
PCR (DNA Viruses) Murine Cytomegalovirus Spleen, Salivary Gland
PCR (DNA Viruses) Ectromelia virus Spleen, Skin lesions
PCR (DNA Viruses) Polyoma Virus Skin, Mammary gland
RT-PCR (RNA Viruses) Mouse Hepatitis Virus Faeces
RT-PCR (RNA Viruses) Rat Coronavirus/SDAV Salivary, Hardarian gland
RT-PCR (RNA Viruses) Rotavirus (EDIM) Faeces, intestine
RT-PCR (RNA Viruses) Murine encephalomyelitis virus (TMEV) Faeces, intestine
RT-PCR (RNA Viruses) Murine Norovirus (MNV) Faeces, Intestine
RT-PCR (RNA Viruses) Sendai Virus Trachea, Lung
RT-PCR (RNA Viruses) Reovirus Type 3 Liver, Lung, Faeces

*all prices GST exclusive.

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Bacteriology Services 

Please contact us for full details.

Abbrev Description Price*
B-SALM Culture and Identification of Salmonella spp: $33.00
B-ENT Culture for Enteric pathogens (ex Salmonella): Aerobic culture on HB and Maconkey agar, colony selection and sub-culture if necessary, identification by biochemical and morphological profile $30.00
B-RESP Culture for respiratory pathogens (ex Mycoplasma spp) :7-8% CO2 culture on HB and maconkey agar, colony selection and subculture in necessary, identification by ciochemical profile (Microbact) or PCR $30.00

*all prices GST exclusive.

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Parasitology Services 

Please contact us for full details.

Abbrev Description Price*
P-ECTO Examination and tape test of pelage for ectoparasites $12.00
P-CELL Cellophane tape test for pinworm ova $12.00
P-FLOAT Faecal flotation test for detection of intestinal parasites $18.00
P-CRAS Wet mount examination of urine (Rats only) for Trichosomoides crassicauda $12.00

*all prices GST exclusive.

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Pathology and Histopathology Services 

Abbrev Description Price*
PREP Slide preparation and staining (H&E) from fixed tissue samples provided. Interpretation and report. Samples should be submitted in 10% buffered formalin.

Please contact the Melbourne laboratory for advice on organs to be collected and appropriate formalin volumes.
$45.00 per slide
SLIDE Interpretation and report on pre-prepared and stained slides. $25.00 per slide
SPEC Slide preparation and special stain from fixed tissue samples. Interpretation and report. $60.00 per slide

*all prices GST exclusive.

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BTP Serology Kits 

Abbrev Description Price*
SMART-SPT9M Test kit for detection of antibody directed against 9 common pathogens found in mice.

ROTA,MHV,MPUL,MPV,MVM,PVM,REO3,SEND,TMEV

8 spot strips per kit
$484.00
SMART -EIA ELISA Kit for detection of antibody directed against murine Pathogens (96 assays per kit). Contact Cerberus for a full range of kits available. $484.00
SMART-SPT6EM Extended panel kit for detection of antibody in mice directed against

TYZ,ECT,LCM,HAN,MAD1&2,MCMV

8 spot strips per kit
$484.00

*all prices GST exclusive.

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Service Contact Cost
Transgene Copy Number Determination Cerberus P.O.A.
Zygosity Testing Cerberus P.O.A.
Strain Specific Genetic Verification Cerberus P.O.A

 

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Copyright © 2010 Cerberus Sciences.

Cerberus Services

Serology
Serology Panels
Pathology ( Animal Submissions)
Molecular Diagnostics
Bacteriology Services
Parasitology Services
Pathology and Histopathology Services
BTP Serology Kits