Identifying homozygous and hemizygous/heterozygous transgenic animals is important for breeding programs for transgenic colonies. Real-time PCR is used to replace the southern blotting method for zygosity testing. It produces rapid and accurate results for identifying the zygosity status of animals compared with the traditional southern blotting method. Two-fold differences of unknown samples are measured through delta-delta CT analysis compared with the known heterozygous controls.
Examples of Zygosity results